| No. |
Title |
Abstract |
PDF |
PW
083 |
Peptide Analysis by Hydrophilic Interaction Chromatography - IT/TOF Mass Spectrometer (HILIC-LCMS-IT-TOF) on Sub 2-3um Silica Columns
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We have investigated the potential of Hydrophilic Interaction chromatography IT/TOF Mass Spectrometer using the UFLC. The tryptic myoglobin and BSA were well separated on prototype HILIC column.
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2.5MB |
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PW
099 |
Retention Controlling Technique for Cations by Changing Mobile Phase Composition in Non-Suppressed Ion Chromatography |
Non-suppressed ion chromatography is generally more flexible in the selection of the mobile phase. We introduce the retention controlling technique for cations analysis by changing type of acid as mobile phase or addition of mobile phase modifiers. |
2.4MB |
PW
124 |
Some Considerations to Achieve High Quality Ultra High Resolution Analysis Using Ultra Fast Liquid Chromatography
|
The newly developed ultrafast high-resolution HPLC that can perform the analysis without the degradation in the quality of the data, even with higher pressure, is introduced. Evaluation of major performance factors, such as peak area reproducibility, carryover and retention time reproducibility, are demonstrated. |
3.2MB |
PW
125 |
A Novel On-Line Ultra Fast Liquid Chromatography System for Pharmaceutical Process Analytical Technology
|
Process Analytical Technology (PAT) is a key concept for manufacturing processes to improve product quality and process yields. We have developed the on-line HPLC systems with various functions. Each system shows good reproducibility and its potential to apply for PAT.
|
2.1MB |
PT
136 |
Development of the Shimadzu Nanoflow HPLC system, "Prominence nano" |
We introduce the new nanoflow HPLC system which offers great flow-rate precision under very low flow rate. We evaluated nanoflow HPLC and MALDI-TOFMS system performance. Low abundant five proteins were clearly identified based on the Mascot database search result. |
2.1MB |
PT
144 |
Highly Sensitive Analysis of Carbohydrates by Hydrophilic Interaction Chromatography with Postcolumn Fluorometric Detection |
We applied the postcolumn-derivatizing method to HILIC, and optimized the analytical conditions for a separation and a derivatization. We applied this method to the analysis of monosaccharides in brewed foods and obtained good quantitative results through simple and easy pretreatment.
|
2.2MB |
PT
154 |
Explosives Analysis by Ultra-Fast Liquid Chromatography |
Soil samples surrounding current and former munition plants or war areas often show a high level of explosive compounds. A high speed reversed phase method was developed that resolved 17 explosive compounds and surrogates in 14 minutes. |
2.0MB |
PT
156 |
Analysis of Synthetic Polymers using SEC-MALDI-TOFMS
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By combining micro-scale SEC with the AccuSpotTM and MALDI-TOFMS, SEC-MALDI-TOFMS analytical system has been developed. Here, using a copolymer, poly (methylmethacrylate-b-n-butylmethacrylate) , with a complicated composition as a sample, we present an example of detection of a homopolymer present at trace levels in the sample.
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2.6MB |
PT
167 |
Analysis of Haloacetic Acids in Drinking Water by Non-suppressed Ion Chromatography-Mass Spectrometry
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GC and GCMS are commonly used for the analysis of haloacetic acids (HAAs) in drinking water. However, liquid-liquid microextraction and derivatization are required. We tried to analyze HAAs in drinking water by non-suppressed IC hyphenated with MS.
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2.3MB |
PT
179 |
Fragmentation study of catechins in green tea using a solution phase hydrogen/deuterium exchange method and LCMS-IT-TOF tandem mass spectrometry |
In the present research, fragmentation pathways of four catechins have been elucidated. The exact position to form neutral losses of CO2 and C2H2O are confirmed by hydrogen/deuterium exchange experiment and multiple stage mass data.
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3.1MB |
PT
180 |
Screening Analysis of Residual Pesticides in Processed Foods by Accurate Mass Measurement
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The development of a new screening methodology to simultaneously detect hundreds of dangerous chemicals is critical. 111 pesticides standard mixture was analyzed using a LCMS-IT-TOF, and high mass accuracy MSn data were obtained. MS1 measurement enables to achieve the ug/L level sensitivity.
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2.9MB |
PT
182 |
Ultra Fast Liquid Chromatography for the Determination of Flavanones in Citrus Juice
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Ultra Fast Liquid Chromatography which employs sub- or mid-2 um particle size columns has progressed, and the separation equivalent to the conventional method became possible in a fraction of the conventional analysis time. we examined the determination of flavanones in citrus juice
|
2.0MkB |
PT
197 |
Development of a new proteomics platform for non-labeling differential analysis by LCMS combined with a novel nano HPLC system
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We introduce the new nanoflow HPLC system equipped which enables the great reproducibility of retention time. The reproducible platform for non-labeling differential analysis on a LCMS-IT-TOF followed by the PCA（Principal Component Analysis） was developed.
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2.6MB |
PT
198 |
Investigation of LC off-line MALDI system to identify phosphoproteins using nanoLC system and TiO2 method
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NanoLC off-line MALDI system is applicable to analysis of phosphoprotein.The system enables to identify not only phosphoprotein but also phosphorylation sites in single- and multi-phosphorylated peptides using a unique MS/MS configuration, i.e. seamless PSD on CFR.
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2.3MB |
PT
206 |
Profiling of Amine-containing Metabolites Using LC ESI-MS |
We demonstrate that an ion-paired RPHPLC ESI-MS method can be used to separate and detect amine-containing metabolites. Using the retention time of known metabolites and MSn capability, high mass accuracy instruments such as ESI-QIT/TOFMS, identification of metabolites can be achieved with high confidence.
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0.7MB |
PT
211 |
Identification and Determination of Major Constituents in Extracts of Traditional Chinese Medicine Radix Polygoni Multiflori by LCMS-IT-TOF.
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Radix Polygoni Multiflori is an important herbal medicine with diverse biological activities. The high accuracy multi-stage mass data acquried by LCMS-IT-TOF combined with PDA makes the whole system a strong tool of qualitation and quantitation, which is a efficient way for quality control work of Chinese Medicine.
|
3.5MB |
PT
223 |
Fast Determination of Phenolic Compounds in Different Traditional Chinese Medicines by a UFLC Method |
Phenolic compounds are found in various plants including traditional Chinese medicines (TCM). The conventional method with a run time of 100 minutes for profiling and determination of the compositions of phenolic compounds in TCM can be replaced by a UFLC method. The run time was reduced to 13.5 minutes. |
2.1MB |
PT
228 |
Direct LC/MS Analysis of Basic Drugs in Plasma by On-line Pretreatment with Weak Cation-exchange Pretreatment Column
|
The Shim-pack MAYI-WCX column provided the good advantage of matrices clean-up in bio-samples. Basic drugs trapped on the Shim-pack MAYI-WCX could be eluted at conditions of weak acid mobile phase. Shim-pack MAYI-WCX has high compatibility with MS spec. |
3.3MB |
PT
229 |
Identification of Iminium Ion Reactive Intermediates Using High Mass Accuracy MSn Analysis
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This paper describes the application of high resolution isotope filtering to the analysis of in vitro stable oxidative metabolites and 13C and 15N labeled iminium ion reactive intermediates of nefazodone and prochloperazine. |
2.1MB |
PT
230 |
Ultra Fast Liquid Chromatography for the Determination of Ginsenosides in Panax ginseng with Ether-linked Phenyl Column
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Panax ginseng is one of the most useful Chinese herbal medicines. We present a high-speed and high-resolution HPLC method for the simultaneous determination of five major ginsenosides in Panax ginseng using shorter columns packed with smaller sized particles. |
2.4MB |
PT
233 |
Study of ultra fast analysis of pharmaceutical products using LC-MS
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We investigated the analytical conditions of CAs and a CCR. We could gain mass chromatograms with sharp peaks under high flow rate. we have achieved high throughput analysis using UFLC and LCMS-2020 which has the feature of ultra fast scanning and positive/negative switching technique.
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2.1MB |
PT
252 |
Improved technique of pretreatment method for environmental water analysis using column switching LC-MS
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Ion suppression observed in surface water analysis is often caused by humic compounds. We found it possible to solve these problems by employing column switching LC-MS which consists of a pretreatment column containing surface modified polymer particles and 3-valves for cleaning in the pretreatment column.
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3.8MB |
PT
253 |
Profiling of tryptophan metabolites by high speed and high resolution HPLC with an automated column switching pretreatment system
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Tryptophan and its major metabolites were simultaneously determined within ten minutes by a high speed and high resolution HPLC using small particle column with column switching automated deproteinization.
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2.1MB |
PT
254 |
Determination of Impurities in Pharmaceutical Products by Online Pre-treatment System Combined with LCMS-IT-TOF mass spectrometer |
An eluent containing non-volatile salt buffer and ion pair reagent is frequently used, which is not compatible for ESI-MS. We developed an automated online concentration system, which is based on the technique of a 2D-HPLC. The target impurity in the fraction is trapped on the desalting column followed by online loading into a LCMS-IT-TOF. |
2.0MB |
